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Flow cytometric analysis of HeLa cells using β-Actin (D6A8) Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody.
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Western blotting analysis of extracts from various human, mouse, and rat tissues using α-Smooth Muscle Actin (D4K9N) XP ® Rabbit mAb (upper) and GAPDH #5174 (lower). As expected, skeletal muscle samples are negative for α-smooth muscle actin.
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